IIT Guwahati’s New Microfluidic System Boosts Crop Growth
Researchers at the Indian Institute of Technology (IIT) Guwahati have developed a portable, cost-effective microfluidic system designed to replicate soil-like conditions. This system has demonstrated that optimizing nutrient flow can improve root growth and nitrogen uptake, leading to enhanced crop yields.
Innovative Research by IIT Guwahati
Prof Pranab Kumar Mondal, Associate Professor in the Department of Mechanical Engineering and an associate faculty member in the School of Agro and Rural Technology at IIT Guwahati, and his team used microfluidics to understand how the primary root emerging from a seed absorbs nutrients from the soil. This innovative use of microfluidic technology to analyze root behavior holds the potential to significantly enhance crop management and boost agricultural yields by optimizing nutrient delivery and root development in practical farming applications.
Support and Publication
The research work was supported by the Science and Engineering Research Board (SERB/ANRF), Government of India. The team’s findings have been published in the prestigious journal, ‘Lab on a Chip,’ published by the Royal Society of Chemistry, in a paper co-authored by Kaushal Agarwal, Dr Sumit Kumar Mehta, and Prof Pranab Kumar Mondal. The work has also been accepted to be featured as cover art for the upcoming issue of the journal.
Understanding Root Dynamics
The primary root of a germinating seed serves as the plant’s anchor, crucial for absorbing water and nutrients. This root must navigate various soil conditions during early growth, a critical phase for plant survival. Factors such as nutrient supply, pH levels, soil composition, aeration, and temperature significantly influence root development. However, studying root dynamics has been challenging due to the limitations of traditional experimental setups, which often require large containers and complex handling.
Microfluidics and Plant Growth
Microfluidics is the study of fluid flow in micrometre-sized structures. The study has revolutionized research in cell studies by enabling precise control and characterization of fluid dynamics at small scales. Existing microdevices primarily focus on phenomena like root-bacteria interactions, hormonal signaling, and pollen tube growth, with limited exploration into real-time plant root dynamics. Specifically, the impact of mechanical stimuli from nutrient flow on root growth and thigmomorphogenesis (the response of plants to mechanical stress) has not been extensively studied.
Research Findings
To address these challenges, Prof Mondal and his team investigated the high-yielding mustard variety, Pusa Jai Kisan, known for its effective root diameter in the micrometre range. Their goal was to understand how different nutrient flow conditions influence root growth and nitrogen uptake during the critical post-germination stages. Speaking about the research, Dr Mondal said, “Our study provides new insights into plant root dynamics through the use of microfluidic devices. We validated our setup’s design and findings by simulating nutrient flow, measuring nitrogen uptake, and analyzing the effects of nutrient uptake and fluid pressure on root cells. This research enhances our understanding of how mechanical stimuli and nutrient uptake interact, with practical implications for agriculture.”
The researchers found that increasing the flow rate of the nutrient medium enhanced root length and nitrogen uptake up to an optimal rate. Beyond this point, excessive flow-induced stress reduced root length. This research highlights that carefully managed nutrient flow induces significant morphological changes in the root, promoting plant growth. Looking ahead, the team plans to explore the molecular mechanisms underlying flow-induced changes in root growth.
Doubts Revealed
IIT Guwahati -: IIT Guwahati is a famous engineering college in India where students learn and do research in science and technology.
Microfluidic system -: A microfluidic system is a tiny device that can control the flow of very small amounts of liquids, like how water moves through tiny tubes.
Nutrient flow -: Nutrient flow means how nutrients, which are like food for plants, move through the soil to reach the plant roots.
Nitrogen uptake -: Nitrogen uptake is how plants absorb nitrogen from the soil, which helps them grow strong and healthy.
Lab on a Chip -: Lab on a Chip is a scientific journal where researchers publish their new discoveries and inventions.
Mustard variety Pusa Jai Kisan -: Pusa Jai Kisan is a type of mustard plant that farmers grow for its seeds, which are used to make mustard oil and spices.
Science and Engineering Research Board -: The Science and Engineering Research Board is a part of the Indian government that gives money to scientists to help them do important research.